Composite
Part:BBa_K2447016:Design
Designed by: Chee Wai Kit (david) Group: iGEM17_NUS_Singapore (2017-10-11)
Phosphate-dependent, temperature-sensitive cascaded system with IM2 expression
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1026
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2085
Illegal XhoI site found at 1311 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1614
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Silent mutations were introduced to be RFC[10] compatible
Source
TlpA, a transcriptional autorepressor from the virulence plasmid of Salmonella typhimurium.
http://www.nature.com/nchembio/journal/v13/n1/full/nchembio.2233.html?WT.feed_name=subjects_synthetic-biology